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Western Blot analysis of HuvEc cells using NOL10 Polyclonal Antibody
Western Blot analysis of HuvEc cells using NOL10 Polyclonal Antibody
Western Blot analysis of HuvEc cells using NOL10 Polyclonal Antibody

NOL10 Antibody

CSB-PA003462
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNOL10
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Overview

  • Supplier
    Cusabio
  • Product Name
    NOL10 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79954
  • Target name
    NOL10
  • Target description
    nucleolar protein 10
  • Target synonyms
    PQBP5, nucleolar protein 10, H_NH0074G24.1, polyglutamine binding protein 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BSC4
  • Protein Name
    Nucleolar protein 10
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NOL10 using anti-NOL10 antibody (A14358-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human U937 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOL10 antigen affinity purified polyclonal antibody (Catalog # A14358-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOL10 at approximately 80 kDa. The expected band size for NOL10 is at 80 kDa.
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