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NOL9 Antibody

PACO15735
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNOL9
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Overview

  • Supplier
    Assay Genie
  • Product Name
    NOL9 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID79707
  • Target name
    NOL9
  • Target description
    nucleolar protein 9
  • Target synonyms
    Grc3, NET6, polynucleotide 5'-hydroxyl-kinase NOL9, polynucleotide 5'-kinase
  • Host
    Rabbit
  • Protein IDQ5SY16
  • Protein Name
    Polynucleotide 5'-hydroxyl-kinase NOL9
  • Scientific Description
    NOL9 Antibody is a premium polyclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ul format to meet your experimental needs. For optimal performance, store at -20°C or -80°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of NOL9 using anti-NOL9 antibody (A11105-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOL9 antigen affinity purified polyclonal antibody (A11105-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOL9 at approximately 79 kDa. The expected band size for NOL9 is at 79 kDa.
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