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ProductsBack/NOP58 antibody [N1N3]

NOP58 antibody [N1N3]

GTX116419
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetNOP58
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Overview

  • Supplier
    GeneTex
  • Product Name
    NOP58 antibody [N1N3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51602
  • Target name
    NOP58
  • Target description
    NOP58 ribonucleoprotein
  • Target synonyms
    HSPC120, NOP5, NOP5/NOP58, nucleolar protein 58, NOP58 ribonucleoprotein homolog, nucleolar protein 5, nucleolar protein NOP5/NOP58
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y2X3
  • Protein Name
    Nucleolar protein 58
  • Scientific Description
    Required for 60S ribosomal subunit biogenesis.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NOP58 using anti-NOP58 antibody (A05070-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human HEL whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human U251 whole cell lysates, Lane 6: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOP58 antigen affinity purified polyclonal antibody (Catalog # A05070-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOP58 at approximately 70 kDa. The expected band size for NOP58 is at 60 kDa.
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