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ProductsBack/NOSIP antibody [N1C3]
Sample (30 ug of whole cell lysate) A: 293T B: A431 (GTX27909) 12% SDS PAGE GTX116126 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: 293T B: A431 (GTX27909) 12% SDS PAGE GTX116126 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: 293T B: A431 (GTX27909) 12% SDS PAGE GTX116126 diluted at 1:1000

NOSIP antibody [N1C3]

GTX116126
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetNOSIP
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Overview

  • Supplier
    GeneTex
  • Product Name
    NOSIP antibody [N1C3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.53 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID51070
  • Target name
    NOSIP
  • Target description
    nitric oxide synthase interacting protein
  • Target synonyms
    CGI-25, nitric oxide synthase-interacting protein, E3 ubiquitin-protein ligase NOSIP, RING-type E3 ubiquitin transferase NOSIP, eNOS-interacting protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9Y314
  • Protein Name
    Nitric oxide synthase-interacting protein
  • Scientific Description
    Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NOSIP using anti-NOSIP antibody (A07558-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human A431 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOSIP antigen affinity purified polyclonal antibody (Catalog # A07558-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOSIP at approximately 35 kDa. The expected band size for NOSIP is at 35 kDa.
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