Notch1 Antibody (OASE00278)

Aviva Systems Biology

Notch1 Antibody (OASE00278)

23

ImmunoFluorescence, Western Blot, ImmunoCytoChemistry

Research Use Only

Monoclonal

S253-32

1 mg/ml

Unconjugated

Liquid. PBS pH 7.4, 50% glycerol, 0.09% sodium azide

Notch1

notch 1

9930111A19Rik; lin; lin-12; major type A protein; Mi; Mis6; Motch A; mT14; N; N1; neurogenic locus notch homolog protein 1; Notch gene homolog 1; p300; Ta; Tan1

Mouse

IgG1

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane.

Human, Mouse, Rat

-20°C

12352203