More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/NOX2 / gp91phox antibody
WB analysis of various samples using GTX12024 NOX2 / gp91phox antibody. Lane 1 : rat thymus tissue lysates Lane 2 : rat brain tissue lysates Dilution : 0.5 μg/mL Loading : 50μg of sample under reducing conditions
WB analysis of various samples using GTX12024 NOX2 / gp91phox antibody. Lane 1 : rat thymus tissue lysates Lane 2 : rat brain tissue lysates Dilution : 0.5 μg/mL Loading : 50μg of sample under reducing conditions
WB analysis of various samples using GTX12024 NOX2 / gp91phox antibody. Lane 1 : rat thymus tissue lysates Lane 2 : rat brain tissue lysates Dilution : 0.5 μg/mL Loading : 50μg of sample under reducing conditions

NOX2 / gp91phox antibody

GTX12024
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetCYBB
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    NOX2 / gp91phox antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. IHC-P: 0.5-1microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID1536
  • Target name
    CYBB
  • Target description
    cytochrome b-245 beta chain
  • Target synonyms
    AMCBX2, CGD, CGDX, GP91-1, GP91-PHOX, GP91PHOX, IMD34, NOX2, p91-PHOX, NADPH oxidase 2, CGD91-phox, cytochrome b(558) subunit beta, cytochrome b-245 beta polypeptide, cytochrome b-245 heavy chain, cytochrome b558 subunit beta, heme-binding membrane glycoprotein gp91phox, neutrophil cytochrome b 91 kDa polypeptide, p22 phagocyte B-cytochrome, superoxide-generating NADPH oxidase heavy chain subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP04839
  • Protein Name
    NADPH oxidase 2
  • Scientific Description
    Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cells respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Wei X, Xue M, Kang C, et al. Increased NOX1 and DUOX2 expression in the colonic mucosa of patients with chronic functional constipation. Medicine (Baltimore). 2022,101(32):e30028. doi: 10.1097/MD.0000000000030028
    Read this paper

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Absolute Antibody
Anti-NADPH oxidase 2 [NL7]AB03684-1.1
ApplicationsFlow Cytometry, Western Blot, Neutralisation/Blocking
ReactivityHuman
TargetCYBB
  • SizePrice
Product group Antibodies
RayBiotech
Anti-NOX2/gp91phox Antibody144-62329
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCYBB
  • SizePrice
Figure 1. Western blot analysis of NOX2/gp91phox/CYBB using anti-NOX2/gp91phox/CYBB antibody (A00328-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HELA whole cell lysates, Lane 2: human HEPG2 whole cell lysates, Lane 3: human U937 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX2/gp91phox/CYBB antigen affinity purified polyclonal antibody (Catalog # A00328-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX2/gp91phox/CYBB at approximately 65KD. The expected band size for NOX2/gp91phox/CYBB is at 65KD.
Product group Antibodies
Boster Bio
Anti-NOX2/gp91phox/CYBB Antibody Picoband(r)A00328-3-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetCYBB
  • SizePrice
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX2 / gp91phox antibody (GTX133715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
References
GeneTex
NOX2 / gp91phox antibodyGTX133715
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCYBB
  • SizePrice
IHC-P analysis of rat bone marrow tissue using GTX00785 NOX2 / gp91phox antibody. Dilution : 1:200
Product group Antibodies
GeneTex
NOX2 / gp91phox antibodyGTX00785
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCYBB
  • SizePrice
WB analysis of various samples using GTX01533 NOX2 / gp91phox antibody [GT1225]. Dilution : 1:1000 Loading : 25 μg
Product group Antibodies
GeneTex
NOX2 / gp91phox antibody [GT1225]GTX01533
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCYBB
  • SizePrice
Product group Antibodies
Biomatik
Cybb Polyclonal AntibodyCAC08347
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetCYBB
  • SizePrice
Anti-CYBB Antibody
Product group Antibodies
Antibodies.com
Anti-CYBB AntibodyA101483
ApplicationsWestern Blot, ELISA
ReactivityHuman
  • SizePrice
IHC-P analysis of human placenta using GTX88404 NOX2 / gp91phox antibody, Internal. Antigen retrieval : citrate buffer pH 6 Dilution : 3.8μg/ml
Product group Antibodies
GeneTex
NOX2 / gp91phox antibody, InternalGTX88404
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCYBB
  • SizePrice
IHC-P analysis of rat brain tissue using GTX31930 NOX2 / gp91phox antibody. Green : Primary antibody Blue : DAPI Dilution : 20 μg/ml
Product group Antibodies
GeneTex
NOX2 / gp91phox antibodyGTX31930
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCYBB
  • SizePrice