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IHC-P analysis of rat kidney tissue using GTX12205 NOX4 antibody. Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins Dilution : 1μg/ml
IHC-P analysis of rat kidney tissue using GTX12205 NOX4 antibody. Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins Dilution : 1μg/ml
IHC-P analysis of rat kidney tissue using GTX12205 NOX4 antibody. Antigen retireval : Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins Dilution : 1μg/ml

NOX4 antibody

GTX12205
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNox4
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Overview

  • Supplier
    GeneTex
  • Product Name
    NOX4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.1-0.5microg/ml. ICC/IF: 0.5-1microg/ml. IHC-P: 0.5-1microg/ml. IHC-Fr: 0.5-1microg/ml. FCM: 1-3microg/1x106cells. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID50490
  • Target name
    Nox4
  • Target description
    NADPH oxidase 4
  • Target synonyms
    NADPH oxidase 4, KOX-1, kidney oxidase-1, kidney superoxide-producing NADPH oxidase, renal NAD(P)H-oxidase, superoxide-generating NADPH oxidase 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9JHI8
  • Protein Name
    NADPH oxidase 4
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Product group Antibodies
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Figure 1. Western blot analysis of NADPH oxidase 4/NOX4 using anti-NADPH oxidase 4/NOX4 antibody (PA1929). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human HK-2 whole cell lysates, Lane 4: human HL-60 whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human SW579 whole cell lysates, Lane 7: human SK-OV-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NADPH oxidase 4/NOX4 antigen affinity purified polyclonal antibody (Catalog # PA1929) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NADPH OXIDASE 4/NOX4 at approximately 67KD. The expected band size for NADPH OXIDASE 4/NOX4 is at 67KD.
Product group Antibodies
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Anti-NADPH oxidase 4/NOX4 Antibody Picoband(r)PA1929-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
ReactivityBovine, Human, Mouse, Rat
TargetNox4
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