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Western Blot analysis of Jurkat cells using Nox5 Polyclonal Antibody
Western Blot analysis of Jurkat cells using Nox5 Polyclonal Antibody
Western Blot analysis of Jurkat cells using Nox5 Polyclonal Antibody

NOX5 Antibody

CSB-PA060144
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNOX5
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Overview

  • Supplier
    Cusabio
  • Product Name
    NOX5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID79400
  • Target name
    NOX5
  • Target description
    NADPH oxidase 5
  • Target synonyms
    NADPH oxidase 5; NADPH oxidase, EF-hand calcium binding domain 5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96PH1
  • Protein Name
    NADPH oxidase 5
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NOX5 using anti-NOX5 antibody (A03090). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysate, Lane 2: human placenta tissue lysates, Lane 3: human SGC-7901 whole cell lysate, Lane 4: human Jurkat whole cell lysate, Lane 5: human THP-1 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOX5 antigen affinity purified polyclonal antibody (Catalog # A03090) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOX5 at approximately 86KD. The expected band size for NOX5 is at 86KD.
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