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Western blot All lanes: NOXA1 antibody at 2 ug/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 52, 46 kDa Observed band size: 51 kDa
Western blot All lanes: NOXA1 antibody at 2 ug/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 52, 46 kDa Observed band size: 51 kDa
Western blot All lanes: NOXA1 antibody at 2 ug/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 51, 52, 46 kDa Observed band size: 51 kDa

NOXA1 Antibody

CSB-PA015963LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNOXA1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NOXA1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10811
  • Target name
    NOXA1
  • Target description
    NADPH oxidase activator 1
  • Target synonyms
    NY-CO-31, SDCCAG31, p51NOX, NADPH oxidase activator 1, NCF2-like protein, Nox activator 1, antigen NY-CO-31, inhibitory NADPH oxidase activator 1, p51-nox, p67phox-like factor, serologically defined colon cancer antigen 31
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ86UR1
  • Protein Name
    NADPH oxidase activator 1
  • Scientific Description
    Functions as an activator of NOX1, a superoxide-producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NOXA1 using anti-NOXA1 antibody (A04679). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NOXA1 antigen affinity purified polyclonal antibody (Catalog # A04679) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NOXA1 at approximately 60 kDa. The expected band size for NOXA1 is at 51 kDa.
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