NPEPPS antibody [HL1531] detects NPEPPS protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NPEPPS stained by NPEPPS antibody [HL1531] (GTX637005) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
![Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637005/GTX637005_T-44690_20220624_WB_M_R_22062919_101.webp "Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637005/GTX637005_44753_20220729_WB_22080119_658.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![NPEPPS antibody [HL1531] detects NPEPPS protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NPEPPS stained by NPEPPS antibody [HL1531] (GTX637005) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637005/GTX637005_44753_20220819_IHC-P_M_22090701_643.webp "NPEPPS antibody [HL1531] detects NPEPPS protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NPEPPS stained by NPEPPS antibody [HL1531] (GTX637005) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![NPEPPS antibody [HL1531] detects NPEPPS protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: NPEPPS stained by NPEPPS antibody [HL1531] (GTX637005) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX637005/GTX637005_44753_20221021_ICC_IF_22110201_861.webp "NPEPPS antibody [HL1531] detects NPEPPS protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: NPEPPS stained by NPEPPS antibody [HL1531] (GTX637005) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637005/GTX637005_44753_20221209_WB_D_C_22121123_946.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole zebrafish extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637005/GTX637005_44753_20221216_WB_Z_22122018_765.webp "Whole zebrafish extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NPEPPS antibody [HL1531] (GTX637005) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
NPEPPS antibody [HL1531] detects NPEPPS protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. NPEPPS stained by NPEPPS antibody [HL1531] (GTX637005) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
NPEPPS antibody [HL1531]
GTX637005
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Feline, Human, Mouse, Rat, Zebra Fish
TargetNPEPPS
Overview
- SupplierGeneTex
- Product NameNPEPPS antibody [HL1531]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1531
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID9520
- Target nameNPEPPS
- Target descriptionaminopeptidase puromycin sensitive
- Target synonymsAAP-S, MP100, PSA, puromycin-sensitive aminopeptidase, cytosol alanyl aminopeptidase, metalloproteinase MP100
- HostRabbit
- IsotypeIgG
- Protein IDP55786
- Protein NamePuromycin-sensitive aminopeptidase
- Scientific DescriptionThis gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle. [provided by RefSeq, Jul 2008]
- ReactivityCanine, Feline, Human, Mouse, Rat, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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