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ProductsBack/NPFF2 / NPFFR2 Antibody

NPFF2 / NPFFR2 Antibody

LS-C497373
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNPFFR2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NPFF2 / NPFFR2 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 15kDa/48kDa/49kDa/60kDa, while the observed MW by Western blot was 48kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB The predicted MW is 15kDa/48kDa/49kDa/60kDa, while the observed MW by Western blot was 48kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10886
  • Target name
    NPFFR2
  • Target description
    neuropeptide FF receptor 2
  • Target synonyms
    GPR74, HLWAR77, NPFF2, NPGPR, neuropeptide FF receptor 2, G-protein coupled receptor 74, G-protein coupled receptor HLWAR77, neuropeptide FF 2, neuropeptide G-protein coupled receptor
  • Host
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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IHC image of CSB-PA015983NA01HU diluted at 1:500 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of NPFFR2 using anti-NPFFR2 antibody (A08389-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPFFR2 antigen affinity purified polyclonal antibody (Catalog # A08389-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPFFR2 at approximately 60 kDa. The expected band size for NPFFR2 is at 60 kDa.
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