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Western Blot analysis of Jurkat K562 cells using NPRL2 Polyclonal Antibody
Western Blot analysis of Jurkat K562 cells using NPRL2 Polyclonal Antibody
Western Blot analysis of Jurkat K562 cells using NPRL2 Polyclonal Antibody

NPRL2 Antibody

CSB-PA004730
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNPRL2
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Overview

  • Supplier
    Cusabio
  • Product Name
    NPRL2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10641
  • Target name
    NPRL2
  • Target description
    NPR2 like, GATOR1 complex subunit
  • Target synonyms
    FFEVF2, NPR2, NPR2L, TUSC4, GATOR1 complex protein NPRL2, 2810446G01Rik, G21 protein, GATOR complex protein NPRL2, NPR2-like protein, gene 21 protein, homologous to yeast nitrogen permease (candidate tumor suppressor), mutant NPRL2, nitrogen permease regulator 2-like protein, tumor suppressor candidate 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ8WTW4
  • Protein Name
    GATOR1 complex protein NPRL2
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NPRL2 using anti-NPRL2 antibody (A06286-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat skeletal muscle tissue lysates, Lane 2: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPRL2 antigen affinity purified polyclonal antibody (A06286-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPRL2 at approximately 48 kDa. The expected band size for NPRL2 is at 44 kDa.
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