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NPTXR / NPR Antibody

LS-C780763
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNPTXR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NPTXR / NPR Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    ELISA (1:5000 - 1:20000), WB (1:500 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID23467
  • Target name
    NPTXR
  • Target description
    neuronal pentraxin receptor
  • Target synonyms
    NPR, neuronal pentraxin receptor
  • Host
    Rabbit
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NPTXR using anti-NPTXR antibody (A15505-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U87 whole cell lysates, Lane 2: human SiHa whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NPTXR antigen affinity purified polyclonal antibody (Catalog # A15505-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NPTXR at approximately 66 kDa. The expected band size for NPTXR is at 53 kDa.
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Anti-NPTXR Antibody Picoband(r)A15505-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetNPTXR
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Immunohistochemistry analysis in human cerebral cortex and liver tissues using HPA001079 antibody. Corresponding NPTXR RNA-seq data are presented for the same tissues.
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Atlas Antibodies
Anti-NPTXR AntibodyHPA001079
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ReactivityHuman
TargetNPTXR
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