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Nqo2 Polyclonal Antibody

CAC08680
Biomatik
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetNQO2
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Overview

  • Supplier
    Biomatik
  • Product Name
    NQO2 Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IF:1:50-1:200, IP:1:200-1:2000
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4835
  • Target name
    NQO2
  • Target description
    N-ribosyldihydronicotinamide:quinone reductase 2
  • Target synonyms
    DHQV; DIA6; NAD(P)H dehydrogenase, quinone 2; NAD(P)H menadione oxidoreductase-1, dioxin-inducible-2; NAD(P)H quinone dehydrogenase 2; NMOR2; NRH:quinone oxidoreductase 2; QR2; quinone reductase 2; ribosyldihydronicotinamide dehydrogenase; ribosyldihydronicotinamide dehydrogenase [quinone]
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP16083
  • Protein Name
    Ribosyldihydronicotinamide dehydrogenase [quinone]
  • Scientific Description
    The Nqo2 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF, IP.
  • Reactivity Supplier
    Human, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NQO2 using anti-NQO2 antibody (A03112-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NQO2 antigen affinity purified polyclonal antibody (Catalog # A03112-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NQO2 at approximately 26 kDa. The expected band size for NQO2 is at 26 kDa.
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