Paraformaldehyde-fixed, paraffin-embedded mouse spleen tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) incubation at 37°C for 20 minutes; Antibody incubation with NROB2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
NR0B2 Polyclonal Antibody
BS-4311R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat
TargetNR0B2
Overview
- SupplierBioss Antibodies
- Product NameNR0B2 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID8431
- Target nameNR0B2
- Target descriptionnuclear receptor subfamily 0 group B member 2
- Target synonymsnuclear receptor SHP; nuclear receptor subfamily 0 group B member 2; orphan nuclear receptor SHP; SHP; SHP1; small heterodimer partner
- HostRabbit
- IsotypeIgG
- Protein IDQ15466
- Protein NameNuclear receptor subfamily 0 group B member 2
- ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Fish oil alleviates circadian bile composition dysregulation in male mice with NAFLD. Liu Y et al., 2019 Jul, J Nutr BiochemRead more