
Paraformaldehyde-fixed, paraffin-embedded mouse spleen tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15 minutes; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) incubation at 37°C for 20 minutes; Antibody incubation with NROB2 Polyclonal Antibody, Unconjugated (bs-4311R) at 1:200 overnight at 4°C, followed by a conjugated secondary antibody and DAB staining.
NR0B2 Polyclonal Antibody
BS-4311R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat
TargetNR0B2
Overview
- SupplierBioss
- Product NameNR0B2 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 ug/ul
- ConjugateUnconjugated
- Gene ID8431
- Target nameNR0B2
- Target descriptionnuclear receptor subfamily 0 group B member 2
- Target synonymsSHP, SHP1, nuclear receptor subfamily 0 group B member 2, nuclear receptor SHP, orphan nuclear receptor SHP, small heterodimer partner
- HostRabbit
- IsotypeIgG
- Protein IDQ15466
- Protein NameNuclear receptor subfamily 0 group B member 2
- ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat
- Storage Instruction-20°C
- UNSPSC12352203
References
- Liu Y, Li Q, Wang H, et al. Fish oil alleviates circadian bile composition dysregulation in male mice with NAFLD. J Nutr Biochem. 2019,69:53-62. doi: 10.1016/j.jnutbio.2019.03.005Read this paper


![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with NR0B2 antibody [N2C3] (GTX113874) diluted at 1:500.](https://www.genetex.com/upload/website/prouct_img/normal/GTX113874/GTX113874_40618_20160107_WB_B_w_23060501_147.webp)

