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Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: NR1H4 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 55, 49 kDa Observed band size: 56 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: NR1H4 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 55, 49 kDa Observed band size: 56 kDa
Western Blot Positive WB detected in: HEK293 whole cell lysate All lanes: NR1H4 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 55, 49 kDa Observed band size: 56 kDa

NR1H4 Antibody

CSB-PA839406LA01HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNR1H4
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Overview

  • Supplier
    Cusabio
  • Product Name
    NR1H4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID9971
  • Target name
    NR1H4
  • Target description
    nuclear receptor subfamily 1 group H member 4
  • Target synonyms
    BAR, FXR, HRR-1, HRR1, PFIC5, RIP14, bile acid receptor, RXR-interacting protein 14, farnesoid X nuclear receptor, farnesoid X-activated receptor, farnesol receptor HRR-1, retinoid X receptor-interacting protein 14
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96RI1
  • Protein Name
    Bile acid receptor
  • Scientific Description
    Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NR1H4 using anti-NR1H4 antibody (A00835-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCT tissue lysates, Lane 2: human HCCP tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NR1H4 antigen affinity purified polyclonal antibody (Catalog # A00835-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NR1H4 at approximately 56 kDa. The expected band size for NR1H4 is at 56 kDa.
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Lane 1: HepG2 lysates probed with Bile Acid Receptor NR1H4 Antibody (bs-12867R) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:10000 for 90 min at 37˚C._x000D_
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