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ProductsBack/NRD1 / Nardilysin Antibody

NRD1 / Nardilysin Antibody

LS-C410654
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetNRDC
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NRD1 / Nardilysin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 131kDa/139kDa, while the observed MW by Western blot was 132kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:200 - 1:2000) The predicted MW is 131kDa/139kDa, while the observed MW by Western blot was 132kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID4898
  • Target name
    NRDC
  • Target description
    nardilysin convertase
  • Target synonyms
    NRD1, hNRD1, hNRD2, nardilysin, NRD convertase, nardilysin (N-arginine dibasic convertase)
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western Blot Positive WB detected in: K562 whole cell lysate, PC-3 whole cell lysate All lanes: NRDC antibody at 3.73microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 132, 140 kDa Observed band size: 132 kDa
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Figure 1. Western blot analysis of NRD1/NRDC using anti-NRD1/NRDC antibody (A05739-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human SIHA whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRD1/NRDC antigen affinity purified polyclonal antibody (Catalog # A05739-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRD1/NRDC at approximately 140 kDa. The expected band size for NRD1/NRDC is at 130 kDa.
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