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Western Blot analysis of K562 cells using NTN Polyclonal Antibody
Western Blot analysis of K562 cells using NTN Polyclonal Antibody
Western Blot analysis of K562 cells using NTN Polyclonal Antibody

NRTN Antibody

CSB-PA006329
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetNRTN
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Overview

  • Supplier
    Cusabio
  • Product Name
    NRTN Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4902
  • Target name
    NRTN
  • Target description
    neurturin
  • Target synonyms
    neurturin; NTN; prepro-neurturin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99748
  • Protein Name
    Neurturin
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Mouse placenta and heart lysates probed with Rabbit Anti-Neurturin Polyclonal Antibody, Unconjugated (bs-0073R) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:500 for 90 min at 37˚C.
Product group Antibodies
References
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Figure 1. Western blot analysis of NRTN using anti-NRTN antibody (A05924-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRTN antigen affinity purified polyclonal antibody (Catalog # A05924-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NRTN at approximately 22 kDa. The expected band size for NRTN is at 22 kDa.
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Anti-NRTN Antibody Picoband(r)A05924-3-CARRIER-FREE
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TargetNRTN
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IHC-P analysis of human brain tissue using GTX85089 Neurturin antibody. Working concentration : 5 microg/ml
Product group Antibodies
GeneTex
Neurturin antibodyGTX85089
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TargetNRTN
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