Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NSD3 antibody [C1C2], Internal (GTX109396) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![NSD3 antibody [C1C2], Internal detects NSD3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NSD3 protein stained by NSD3 antibody [C1C2], Internal (GTX109396) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX109396/GTX109396_41703_20160127_IFA_w_23060500_636.webp "NSD3 antibody [C1C2], Internal detects NSD3 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NSD3 protein stained by NSD3 antibody [C1C2], Internal (GTX109396) diluted at 1:500. Red: phalloidin, a cytoskeleton marker, diluted at 1:200. Scale bar = 10 μm.")
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with NSD3 antibody [C1C2], Internal (GTX109396) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
NSD3 antibody [C1C2], Internal
GTX109396
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNSD3
Overview
- SupplierGeneTex
- Product NameNSD3 antibody [C1C2], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID54904
- Target nameNSD3
- Target descriptionnuclear receptor binding SET domain protein 3
- Target synonymsKMT3F, KMT3G, WHISTLE, WHSC1L1, pp14328, histone-lysine N-methyltransferase NSD3, Wolf-Hirschhorn syndrome candidate 1-like 1, nuclear SET domain-containing protein 3, protein whistle
- HostRabbit
- IsotypeIgG
- Protein IDQ9BZ95
- Protein NameHistone-lysine N-methyltransferase NSD3
- Scientific DescriptionThis gene is related to the Wolf-Hirschhorn syndrome candidate-1 gene and encodes a protein with PWWP (proline-tryptophan-tryptophan-proline) domains. The function of the protein has not been determined. Two alternatively spliced variants have been described. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Yan M, Li L, Wang Q, et al. The Chinese herbal medicine Fufang Zhenzhu Tiaozhi protects against diabetic cardiomyopathy by alleviating cardiac lipotoxicity-induced oxidative stress and NLRP3-dependent inflammasome activation. Biomed Pharmacother. 2022,148:112709. doi: 10.1016/j.biopha.2022.112709Read this paper
- Liu C, Yu Y, Liu F, et al. A chromatin activity-based chemoproteomic approach reveals a transcriptional repressome for gene-specific silencing. Nat Commun. 2014,5:5733. doi: 10.1038/ncomms6733Read this paper
Datasheet
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