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NSFL1C Antibody

LS-C334867
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNSFL1C
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NSFL1C Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 28kDa/37kDa/40kDa, while the observed MW by Western blot was 41kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:50 - 1:200), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 28kDa/37kDa/40kDa, while the observed MW by Western blot was 41kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID55968
  • Target name
    NSFL1C
  • Target description
    NSFL1 cofactor
  • Target synonyms
    P47, UBX1, UBXD10, UBXN2C, dJ776F14.1, NSFL1 cofactor p47, NSFL1 (p97) cofactor (p47), SHP1 homolog, UBX domain-containing protein 2C, p97 cofactor p47
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: NSFL1C antibody at 6.3ug/ml Lane 1: Mouse brain tissue Lane 2: A431 whole cell lysate Lane 3: 293T whole cell lysate Lane 4: Rat liver tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 41, 38, 29 kDa Observed band size: 41, 130 kDa
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Figure 1. Western blot analysis of NSFL1C using anti-NSFL1C antibody (A08116-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: rat RH35 whole cell lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSFL1C antigen affinity purified polyclonal antibody (Catalog # A08116-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSFL1C at approximately 41 kDa. The expected band size for NSFL1C is at 41 kDa.
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