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NSL1 Antibody

CAB7089
Assay Genie
TargetNSL1
Product group Antibodies
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Overview

  • Supplier
    Assay Genie
  • Product Name
    NSL1 Rabbit pAb
  • Delivery Days Customer
    9
  • Applications Supplier
    WB,IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID25936
  • Target name
    NSL1
  • Target description
    NSL1 component of MIS12 kinetochore complex
  • Target synonyms
    C1orf48; DC8; kinetochore-associated protein NSL1 homolog; MIS14; NSL1, MIND kinetochore complex component, homolog; NSL1, MIS12 kinetochore complex component
  • Host
    Rabbit
  • Protein IDQ96IY1
  • Protein Name
    Kinetochore-associated protein NSL1 homolog
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NSL1 using anti-NSL1 antibody (A09341-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat RH-35 whole cell lysates, Lane 3: mouse thymus tissue lysates, Lane 4: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSL1 antigen affinity purified polyclonal antibody (A09341-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSL1 at approximately 32 kDa. The expected band size for NSL1 is at 32 kDa.
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IHC image of CSB-PA839345LA01HU diluted at 1:300 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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