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Nsun2 Polyclonal Antibody

CAC11327
Biomatik
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityMouse, Rat
TargetNSUN2
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Overview

  • Supplier
    Biomatik
  • Product Name
    Nsun2 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:200-1:500, IF:1:50-1:200
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID54888
  • Target name
    NSUN2
  • Target description
    NOP2/Sun RNA methyltransferase 2
  • Target synonyms
    MISU, MRT5, SAKI, TRM4, RNA cytosine C(5)-methyltransferase NSUN2, 5-methycytoisine methyltransferase, Myc-induced SUN-domain-containing protein, NOL1/NOP2/Sun domain family, member 2, NOP2/Sun RNA methyltransferase family member 2, NOP2/Sun domain family, member 2, mRNA cytosine C(5)-methyltransferase, myc-induced SUN domain-containing protein, substrate of AIM1/Aurora kinase B, tRNA (cytosine(34)-C(5))-methyltransferase, tRNA (cytosine-5-)-methyltransferase NSUN2, tRNA cytosine C(5)-methyltransferase, tRNA methyltransferase 4 homolog
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ08J23
  • Protein Name
    RNA cytosine C(5)-methyltransferase NSUN2
  • Scientific Description
    The Nsun2 Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC, IF.
  • Reactivity
    Mouse, Rat
  • Reactivity Supplier
    Human, Rat, Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NSUN2 using anti-NSUN2 antibody (A03878-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN2 antigen affinity purified polyclonal antibody (Catalog # A03878-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSUN2 at approximately 100 kDa. The expected band size for NSUN2 is at 100 kDa.
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