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WB analysis of human muscle tissue using GTX46175 NSUN3 antibody at 0.2-1microg/ml.
WB analysis of human muscle tissue using GTX46175 NSUN3 antibody at 0.2-1microg/ml.
WB analysis of human muscle tissue using GTX46175 NSUN3 antibody at 0.2-1microg/ml.

NSUN3 antibody, C-term

GTX46175
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetNSUN3
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Overview

  • Supplier
    GeneTex
  • Product Name
    NSUN3 antibody, C-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID63899
  • Target name
    NSUN3
  • Target description
    NOP2/Sun RNA methyltransferase 3
  • Target synonyms
    COXPD48, MST077, MSTP077, tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial, NOL1/NOP2/Sun domain family member 3, NOP2/Sun RNA methyltransferase family member 3, putative methyltransferase NSUN3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H649
  • Protein Name
    tRNA (cytosine(34)-C(5))-methyltransferase, mitochondrial
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Delaunay S, Pascual G, Feng B, et al. Mitochondrial RNA modifications shape metabolic plasticity in metastasis. Nature. 2022,607(7919):593-603. doi: 10.1038/s41586-022-04898-5
    Read this paper
  • Gkatza NA, Castro C, Harvey RF, et al. Cytosine-5 RNA methylation links protein synthesis to cell metabolism. PLoS Biol. 2019,17(6):e3000297. doi: 10.1371/journal.pbio.3000297
    Read this paper

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Figure 1. Western blot analysis of NSUN3 using anti-NSUN3 antibody (A16002). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: monkey COS-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN3 antigen affinity purified polyclonal antibody (Catalog # A16002) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSUN3 at approximately 38 kDa. The expected band size for NSUN3 is at 38 kDa.
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