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ProductsBack/NSUN7 Antibody (HRP)

NSUN7 Antibody (HRP)

LS-C399442
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetNSUN7
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NSUN7 Antibody (HRP)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    HRP
  • Estimated Purity
    ...
  • Gene ID79730
  • Target name
    NSUN7
  • Target description
    NOP2/Sun RNA methyltransferase family member 7
  • Target synonyms
    NOL1/NOP2/Sun domain family member 7; NOP2/Sun domain family, member 7; putative methyltransferase NSUN7
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NSUN7 using anti-NSUN7 antibody (A14267). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: rat liver tissue lysates, Lane 3: rat testis tissue lysates, Lane 4: rat RH-35 whole cell lysates, Lane 5: mouse liver tissue lysates, Lane 6: mouse testis tissue lysates, Lane 7: mouse RAW264.7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NSUN7 antigen affinity purified polyclonal antibody (A14267) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NSUN7 at approximately 81 kDa. The expected band size for NSUN7 is at 81 kDa.
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