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Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA016120ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA016120ESR2HU at dilution of 1:100
Immunohistochemistry of paraffin-embedded human adrenal gland tissue using CSB-PA016120ESR2HU at dilution of 1:100

NTF3 Antibody

CSB-PA016120ESR2HU
Cusabio
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetNTF3
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Overview

  • Supplier
    Cusabio
  • Product Name
    NTF3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4908
  • Target name
    NTF3
  • Target description
    neurotrophin 3
  • Target synonyms
    HDNF; nerve growth factor 2; neurotrophic factor; neurotrophin-3; NGF2; NGF-2; NT3; NT-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP20783
  • Protein Name
    Neurotrophin-3
  • Scientific Description
    Seems to promote the survival of visceral and proprioceptive sensory neurons.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Paraformaldehyde-fixed, paraffin embedded rat brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Neurotrophin 3 Polyclonal Antibody, Unconjugated (bs-0160R ) at 1:200 overnight at 4°C, followed by a conjugated secondary for 90 minutes and DAB staining
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Figure 1. Western blot analysis of Neurotrophin 3/NTF3 using anti-Neurotrophin 3/NTF3 antibody (A03348-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human U87 whole cell lysates, Lane 4: mouse Neuro-2a whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurotrophin 3/NTF3 antigen affinity purified polyclonal antibody (Catalog # A03348-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurotrophin 3/NTF3 at approximately 34KD. The expected band size for Neurotrophin 3/NTF3 is at 34KD.
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WB analysis of various samples using GTX81308 Neurotrophin 3 antibody, C-term. Lane 1: Human liver lysate Lane 2: Human ovary lysate Loading : 20 microg per lane Dilution : 1:2000
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Immunohistochemical staining of human caudate shows strong cytoplasmic positivity in glial cells.
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