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ProductsBack/NTHL1 Antibody
Western Blot Positive WB detected in: NIH/3T3 whole cell lysate All lanes: NTHL1 antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 34, 33 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: NIH/3T3 whole cell lysate All lanes: NTHL1 antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 34, 33 kDa Observed band size: 35 kDa
Western Blot Positive WB detected in: NIH/3T3 whole cell lysate All lanes: NTHL1 antibody at 4ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 35, 34, 33 kDa Observed band size: 35 kDa

NTHL1 Antibody

CSB-PA016125LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetNTHL1
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Overview

  • Supplier
    Cusabio
  • Product Name
    NTHL1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4913
  • Target name
    NTHL1
  • Target description
    nth like DNA glycosylase 1
  • Target synonyms
    FAP3, NTH1, OCTS3, hNTH1, endonuclease III-like protein 1, DNA glycoslyase/AP lyase, DNA glycosylase/AP lyase, bifunctional DNA N-glycoslyase/DNA-(apurinic or apyrimidinic site) lyase, bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase, nth endonuclease III-like 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP78549
  • Protein Name
    Endonuclease III-like protein 1
  • Scientific Description
    Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3 to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines. Has also 8-oxo-7,8-dihydroguanine (8-oxoG) DNA glycosylase activity. Acts preferentially on DNA damage opposite guanine residues in DNA. Is able to process lesions in nucleosomes without requiring or inducing nucleosome disruption.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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NTH1 antibody detects NTH1 protein at nucleus by immunofluorescent analysis. Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NTH1 protein stained by NTH1 antibody (GTX103180) diluted at 1:1000. Blue: Hoechst 33342 staining.
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Figure 1. Western blot analysis of NTHL1 using anti-NTHL1 antibody (A03207-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human A549 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NTHL1 antigen affinity purified polyclonal antibody (Catalog # A03207-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NTHL1 at approximately 34 kDa. The expected band size for NTHL1 is at 34 kDa.
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