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WB analysis of MCF-7 cell lysates using GTX16313 NTR1 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of MCF-7 cell lysates using GTX16313 NTR1 antibody. The lane on the right is blocked with the synthesized peptide.
WB analysis of MCF-7 cell lysates using GTX16313 NTR1 antibody. The lane on the right is blocked with the synthesized peptide.

NTR1 antibody

GTX16313
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetNTSR1
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Overview

  • Supplier
    GeneTex
  • Product Name
    NTR1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500~1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4923
  • Target name
    NTSR1
  • Target description
    neurotensin receptor 1
  • Target synonyms
    NTR, neurotensin receptor type 1, NT-R-1, NTR1, NTRH, high-affinity levocabastine-insensitive neurotensin receptor, neurotensin receptor 1 (high affinity)
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30989
  • Protein Name
    Neurotensin receptor type 1
  • Scientific Description
    Neurotensin receptor 1 belongs to the large superfamily of G-protein coupled receptors. NTSR1 mediates the multiple functions of neurotensin, such as hypotension, hyperglycemia, hypothermia, antinociception, and regulation of intestinal motility and secretion. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Lane 1: Lovo cell lysates; Lane 2: MCF-7 cell lysates probed with Neurotensin Receptor 1 Polyclonal Antibody, Unconjugated (bs-12002R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of Neurotensin Receptor 1/NTSR1 using anti-Neurotensin Receptor 1/NTSR1 antibody (A04610-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MCF-7 whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Neurotensin Receptor 1/NTSR1 antigen affinity purified polyclonal antibody (Catalog # A04610-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Neurotensin Receptor 1/NTSR1 at approximately 43 kDa. The expected band size for Neurotensin Receptor 1/NTSR1 is at 43 kDa.
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