Nuclear Antigen Antibody

Biorbyt

Nuclear Antigen Antibody

10

Optimal dilution of the Nuclear Marker antibody should be determined by the researcher.1. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

ImmunoFluorescence, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT IF, IHC-P

Research Use Only

Monoclonal

NM106

Unconjugated

Mouse

IgG1

This mAb is an excellent marker for all nuclei in cells in tissues. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This mAb recognizes an antigen associated with the nuclei in all cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed tissue sections.

Human, Mouse, Rat

-20°C,2°C to 8°C

12352203