Immunohistochemistry analysis of nuclear staining of colon cancer sections using Nucleolin mAb (4E2).
![Western Blot analysis using Nucleolin [4E2] antibody (GTX13541) on HL60 (1) and ZR-75 (2) cell extracts](https://www.genetex.com/upload/website/prouct_img/normal/GTX13541/Nucleolin-antibody-4E2-GTX13541-WB-1_19011509_566.jpeg "Western Blot analysis using Nucleolin [4E2] antibody (GTX13541) on HL60 (1) and ZR-75 (2) cell extracts")
Immunohistochemistry analysis of nuclear staining of colon cancer sections using Nucleolin mAb (4E2).
Nucleolin antibody [4E2]
GTX13541
ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityBovine, Human
TargetNCL
Overview
- SupplierGeneTex
- Product NameNucleolin antibody [4E2]
- Delivery Days Customer9
- Application Supplier NoteICC: Use at a concentration of 1 - 10 microg/ml. IHC: Use at a concentration of 1 - 10 microg/ml. WB: Use at a concentration of 1 microg/ml. Predicted molecular weight: 100 kDa. This antibody allows in situ detection of nucleolar protein in Hep-II cell and paraffin embedded tissue section. Paraffin embedded sections should be processed under microwave treatment. Optimal dilutions/concentrations should be determined by the end user.
- ApplicationsImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID400
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID4691
- Target nameNCL
- Target descriptionnucleolin
- Target synonymsC23; Nsr1; nucleolin
- HostMouse
- IsotypeIgG1
- Protein IDP19338
- Protein NameNucleolin
- Scientific DescriptionNucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]
- ReactivityBovine, Human
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Efficient recovery of the RNA-bound proteome and protein-bound transcriptome using phase separation (OOPS). Villanueva E et al., 2020 Aug, Nat ProtocRead more
- Full-length NF-kappaB repressing factor contains an XRN2 binding domain. Alexandrova J et al., 2020 Feb 28, Biochem JRead more
- Synergistic inhibition of lung cancer cell invasion, tumor growth and angiogenesis using aptamer-siRNA chimeras. Lai WY et al., 2014 Mar, BiomaterialsRead more
- A cis-element with mixed G-quadruplex structure of NPGPx promoter is essential for nucleolin-mediated transactivation on non-targeting siRNA stress. Wei PC et al., 2013 Feb 1, Nucleic Acids ResRead more