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ProductsBack/NUDT12 Rabbit Polyclonal Antibody
Gel: 8%SDS-PAGE Lysate: 40 microg Lane 1-3: 293T cell K562 cell Human pancreas tissue Primary antibody: TA365275 (NUDT12 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds
Gel: 8%SDS-PAGE Lysate: 40 microg Lane 1-3: 293T cell K562 cell Human pancreas tissue Primary antibody: TA365275 (NUDT12 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds
Gel: 8%SDS-PAGE Lysate: 40 microg Lane 1-3: 293T cell K562 cell Human pancreas tissue Primary antibody: TA365275 (NUDT12 Antibody) at dilution 1/250 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

NUDT12 Rabbit Polyclonal Antibody

TA365275
OriGene
ApplicationsWestern Blot
DownloadMSDS
Product group Antibodies
TargetNUDT12
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    NUDT12 Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID83594
  • Target name
    NUDT12
  • Target description
    nudix hydrolase 12
  • Target synonyms
    deNADding enzyme NUDT12; NAD-capped RNA hydrolase NUDT12; NADH pyrophosphatase NUDT12; nucleoside diphosphate linked moiety X-type motif 12; nucleoside diphosphate-linked moiety X motif 12; nudix (nucleoside diphosphate linked moiety X)-type motif 12; nudix motif 12; peroxisomal NADH pyrophosphatase NUDT12
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BQG2
  • Protein Name
    Peroxisomal NADH pyrophosphatase NUDT12
  • Scientific Description
    NUDT12 rabbit polyclonal antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUDT12 using anti-NUDT12 antibody (A13426-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse Hepa1-6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT12 antigen affinity purified polyclonal antibody (A13426-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDT12 at approximately 47 kDa. The expected band size for NUDT12 is at 52 kDa.
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