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NUDT5 Antibody

LS-C497202
LifeSpan BioSciences
ApplicationsWestern Blot
Product group Antibodies
TargetNUDT5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NUDT5 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 24kDa, while the observed MW by Western blot was 36kDa.
  • Applications
    Western Blot
  • Applications Supplier
    WB (1:1000 - 1:2000) The predicted MW is 24kDa, while the observed MW by Western blot was 36kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID11164
  • Target name
    NUDT5
  • Target description
    nudix hydrolase 5
  • Target synonyms
    8-oxo-dGDP phosphatase; ADP-sugar pyrophosphatase; hNUDT5; nuclear ATP-synthesis protein NUDIX5; nudix (nucleoside diphosphate linked moiety X)-type motif 5; YSA1; YSA1H; YSAH1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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IHC image of CSB-PA891535LA01HU diluted at 1:600 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Figure 1. Western blot analysis of NUDT5 using anti-NUDT5 antibody (A07530). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human CACO-2 whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat RH35 whole cell lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse HEPA1/6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUDT5 antigen affinity purified polyclonal antibody (Catalog # A07530) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUDT5 at approximately 35 kDa. The expected band size for NUDT5 is at 35 kDa.
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