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NUFIP1 antibody

GTX118425
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetNUFIP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    NUFIP1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID26747
  • Target name
    NUFIP1
  • Target description
    nuclear FMR1 interacting protein 1
  • Target synonyms
    NUFIP, Rsa1, bA540M5.1, FMR1-interacting protein NUFIP1, NUFIP1, FMR1 interacting protein 1, nuclear FMRP-interacting protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UHK0
  • Protein Name
    FMR1-interacting protein NUFIP1
  • Scientific Description
    Binds RNA.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUFIP1 using anti-NUFIP1 antibody (A10137-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A375 whole cell lysates, Lane 3: human Caco-2 whole cell lysates, Lane 4: human HEL whole cell lysates, Lane 5: human 293T whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUFIP1 antigen affinity purified polyclonal antibody (Catalog # A10137-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUFIP1 at approximately 75 kDa. The expected band size for NUFIP1 is at 75 kDa.
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