More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/NUMB Antibody
Western Blot analysis of A549 cells using NUMB Polyclonal Antibody
Western Blot analysis of A549 cells using NUMB Polyclonal Antibody
Western Blot analysis of A549 cells using NUMB Polyclonal Antibody

NUMB Antibody

CSB-PA005466
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetNUMB
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    NUMB Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8650
  • Target name
    NUMB
  • Target description
    NUMB endocytic adaptor protein
  • Target synonyms
    c14_5527; C14orf41; h-Numb; numb homolog; protein numb homolog; S171
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49757
  • Protein Name
    Protein numb homolog
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

Related products

Product group Antibodies
Everest Biotech
Goat anti-NUMBEB05296
ApplicationsFlow Cytometry, ImmunoFluorescence, ELISA, ImmunoHistoChemistry
TargetNUMB
  • SizePrice
Product group Antibodies
Assay Genie
NUMB AntibodyCAB9352
TargetNUMB
  • SizePrice
Product group Antibodies
Biomatik
Numb Homolog (NUMB) Polyclonal AntibodyCAU21624
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetNUMB
  • SizePrice
Product group Antibodies
GeneTex
NUMB antibody [N1N3]GTX111715
ApplicationsWestern Blot
TargetNUMB
  • SizePrice
Product group Antibodies
MedChem Express
NUMB AntibodyHY-P80253
TargetNUMB
  • SizePrice
Figure 1. Western blot analysis of NUMB using anti-NUMB antibody (A01206-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87 MG whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: rat brain tissue tissue lysates, Lane 6: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUMB antigen affinity purified polyclonal antibody (Catalog # A01206-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUMB at approximately 72-75 kDa. The expected band size for NUMB is at 42 kDa.
Product group Antibodies
Boster Bio
Anti-NUMB Antibody Picoband(r)A01206-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
TargetNUMB
  • SizePrice
Product group Antibodies
Bethyl Laboratories
Rabbit anti-NUMB Antibody, Affinity PurifiedA301-714A
ApplicationsImmunoPrecipitation, Western Blot
TargetNUMB
  • SizePrice
Product group Antibodies
RayBiotech
Anti-NUMB Antibody144-09352
ApplicationsWestern Blot
TargetNUMB
  • SizePrice
Anti-NUMB Antibody
Product group Antibodies
Antibodies.com
Anti-NUMB AntibodyA16316
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
NUMB AntibodyLS-C410886
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
TargetNUMB
  • SizePrice