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NUP62 Polyclonal Antibody

E-AB-60696
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    NUP62 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NUP62 using anti-NUP62 antibody (A03950-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP62 antigen affinity purified polyclonal antibody (Catalog # A03950-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP62 at approximately 69 kDa. The expected band size for NUP62 is at 53 kDa.
Product group Antibodies
Boster Bio
Anti-NUP62 Antibody Picoband(r)A03950-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetNUP62
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Product group Antibodies
RayBiotech
Anti-NUP62 Antibody144-02499
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman
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Product group Antibodies
Biomatik
NUP62 Polyclonal AntibodyCAC13140
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityMouse
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Product group Antibodies
Everest Biotech
Goat anti-NUP62 AntibodyEB06213
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityBovine, Canine, Human
TargetNUP62
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Product group Antibodies
GeneTex
Nucleoporin p62 antibodyGTX102359
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetNUP62
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Product group Antibodies
Assay Genie
NUP62 AntibodyCAB2499
ReactivityHuman
TargetNUP62
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Anti-Nucleoporin p62 Antibody
Product group Antibodies
Antibodies.com
Anti-Nucleoporin p62 AntibodyA326269
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
  • SizePrice
Western Blot Positive WB detected in: Jurkat whole cell lysate, RAW264.7 whole cell lysate, PC-3 whole cell lysate, NIH/3T3 whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate, SH-SY5Y whole cell lysate, K562 whole cell lysate, A549 whole cell lysate All lanes: NUP62 antibody at 3.5ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54 kDa Observed band size: 62 kDa
Product group Antibodies
Cusabio
NUP62 AntibodyCSB-PA016204LA01HU
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetNUP62
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Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Nucleoporin p62 Polyclonal Antibody(bs-2935R) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining.
Product group Antibodies
References
Bioss
Nucleoporin p62 Polyclonal AntibodyBS-2935R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat
TargetNUP62
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Product group Antibodies
MedChem Express
Nucleoporin p62 Antibody (YA2997, Rabbit)HY-P83252
TargetNUP62
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