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Western Blot analysis of K562 cells using Nup88 Polyclonal Antibody
Western Blot analysis of K562 cells using Nup88 Polyclonal Antibody
Western Blot analysis of K562 cells using Nup88 Polyclonal Antibody

NUP88 Antibody

CSB-PA004698
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetNUP88
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Overview

  • Supplier
    Cusabio
  • Product Name
    NUP88 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4927
  • Target name
    NUP88
  • Target description
    nucleoporin 88
  • Target synonyms
    FADS4, nuclear pore complex protein Nup88, karyoporin, nuclear pore complex protein 88, nucleoporin 88kDa, nucleoporin Nup88
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99567
  • Protein Name
    Nuclear pore complex protein Nup88
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of NUP88 using anti-NUP88 antibody (A05382-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: human Jurkat whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP88 antigen affinity purified polyclonal antibody (Catalog # A05382-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP88 at approximately 84 kDa. The expected band size for NUP88 is at 84 kDa.
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