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NUPL1 Antibody

LS-C830630
LifeSpan BioSciences
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNUP58
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    NUPL1 Antibody
  • Delivery Days Customer
    23
  • Applications
    ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA (1:5000 - 1:10000), IHC (1:50 - 1:300)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID9818
  • Target name
    NUP58
  • Target description
    nucleoporin 58
  • Target synonyms
    NUP45, NUPL1, PRO2463, nucleoporin p58/p45, 58 kDa nucleoporin, nucleoporin 58kDa, nucleoporin like 1, nucleoporin-like protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of NUP58 using anti-NUP58 antibody (A09840). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Caco-2 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human HCCT tissue lysates, Lane 5: rat pancreas tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NUP58 antigen affinity purified polyclonal antibody (Catalog # A09840) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NUP58 at approximately 69 kDa. The expected band size for NUP58 is at 61 kDa.
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Western blot All lanes: NUP58 antibody at 4ug/ml Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: LO2 whole cell lysate Lane 4: Mouse thymus tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 61, 51, 60 kDa Observed band size: 61 kDa
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