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WB analysis of SK-N-SH cell lysate using GTX85232 OCIAD2 antibody. Working concentration : (A) 0.5 and (B) 1 microg/ml
WB analysis of SK-N-SH cell lysate using GTX85232 OCIAD2 antibody. Working concentration : (A) 0.5 and (B) 1 microg/ml
WB analysis of SK-N-SH cell lysate using GTX85232 OCIAD2 antibody. Working concentration : (A) 0.5 and (B) 1 microg/ml

OCIAD2 antibody

GTX85232
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetOCIAD2
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Overview

  • Supplier
    GeneTex
  • Product Name
    OCIAD2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.5 - 1 microg/mL. ICC/IF: 20 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID132299
  • Target name
    OCIAD2
  • Target description
    OCIA domain containing 2
  • Target synonyms
    OCIA domain-containing protein 2; ovarian cancer immunoreactive antigen domain containing 2; ovarian carcinoma immunoreactive antigen-like protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ56VL3
  • Protein Name
    OCIA domain-containing protein 2
  • Scientific Description
    OCIAD2 was identified by its sequence similarity with OCIAD1, and together OCIAD1 and OCIAD2 form the OCIA domain family. OCIAD2 mRNA was found to be expressed at higher levels in invasive adenocarcinoma mixed subtype with bronchioloalveolar carcinoma component (BAC) of the lung. Loss of OCIAD2 expression was significantly correlated with lymphatic invasion, blood vessel invasion, and lymph node metastasis, indicating that OCIAD2 may play a role in cell adhesion and prevention of cell migration. While the function of OCIAD2 is still unknown, its expression in adenocarcinoma with BAC component is significantly associated with a favorable prognosis and may serve as a marker for selecting tumors that are treatable by limited surgery.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OCIAD2 using anti-OCIAD2 antibody (A13969-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates, Lane 2: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OCIAD2 antigen affinity purified polyclonal antibody (Catalog # A13969-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OCIAD2 at approximately 17 kDa. The expected band size for OCIAD2 is at 17 kDa.
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