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Western Blot analysis of COLO205 cells using OCRL Polyclonal Antibody
Western Blot analysis of COLO205 cells using OCRL Polyclonal Antibody
Western Blot analysis of COLO205 cells using OCRL Polyclonal Antibody

OCRL Antibody

CSB-PA003502
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetOCRL
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Overview

  • Supplier
    Cusabio
  • Product Name
    OCRL Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4952
  • Target name
    OCRL
  • Target description
    OCRL inositol polyphosphate-5-phosphatase
  • Target synonyms
    DENT2; Dent-2; inositol polyphosphate 5-phosphatase OCRL; inositol polyphosphate 5-phosphatase OCRL-1; INPP5F; LOCR; Lowe oculocerebrorenal syndrome protein; NPHL2; OCRL1; OCRL-1; oculocerebrorenal syndrome of Lowe; phosphatidylinositol 3,4,5-triphosphate 5-phosphatase; phosphatidylinositol polyphosphate 5-phosphatase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ01968
  • Protein Name
    Inositol polyphosphate 5-phosphatase OCRL-1
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OCRL using anti-OCRL antibody (A02042-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OCRL antigen affinity purified polyclonal antibody (Catalog # A02042-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OCRL at approximately 104 kDa. The expected band size for OCRL is at 104 kDa.
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