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ODF2L Antibody

LS-C803533
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetODF2L
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ODF2L Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Peptide-ELISA (1:20000 - 1:40000), WB (1:500 - 1:2000) For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID57489
  • Target name
    ODF2L
  • Target description
    outer dense fiber of sperm tails 2 like
  • Target synonyms
    basal body centriole-associated protein; protein BCAP
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ODF2L using anti-ODF2L antibody (A16444-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U251 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ODF2L antigen affinity purified polyclonal antibody (Catalog # A16444-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ODF2L at approximately 65 kDa. The expected band size for ODF2L is at 74 kDa.
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