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WB analysis of 293 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32004 OGFOD2 antibody. Working concentration : 1 microg/ml
WB analysis of 293 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32004 OGFOD2 antibody. Working concentration : 1 microg/ml
WB analysis of 293 cell lysate in (A) the absence and (B) the presence of blocking peptide using GTX32004 OGFOD2 antibody. Working concentration : 1 microg/ml

OGFOD2 antibody

GTX32004
GeneTex
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetOGFOD2
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Overview

  • Supplier
    GeneTex
  • Product Name
    OGFOD2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1 microg/mL. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID79676
  • Target name
    OGFOD2
  • Target description
    2-oxoglutarate and iron dependent oxygenase domain containing 2
  • Target synonyms
    2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ6N063
  • Protein Name
    2-oxoglutarate and iron-dependent oxygenase domain-containing protein 2
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OGFOD2 using anti-OGFOD2 antibody (A16867-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human K562 whole cell lysates, Lane 4: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OGFOD2 antigen affinity purified polyclonal antibody (Catalog # A16867-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OGFOD2 at approximately 39 kDa. The expected band size for OGFOD2 is at 39,32 kDa.
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