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WB analysis of human small intestine tissue using GTX45080 OLAH antibody at 0.2-1microg/ml.
WB analysis of human small intestine tissue using GTX45080 OLAH antibody at 0.2-1microg/ml.
WB analysis of human small intestine tissue using GTX45080 OLAH antibody at 0.2-1microg/ml.

OLAH antibody, N-term

GTX45080
GeneTex
ApplicationsWestern Blot
Product group Antibodies
TargetOLAH
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Overview

  • Supplier
    GeneTex
  • Product Name
    OLAH antibody, N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 0.2-2.5 ug/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5-1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55301
  • Target name
    OLAH
  • Target description
    oleoyl-ACP hydrolase
  • Target synonyms
    AURA1, SAST, TE2, THEDC1, S-acyl fatty acid synthase thioesterase, medium chain, augmented in rheumatoid arthritis 1, oleoyl-acyl-carrier-protein hydrolase, thioesterase 2, thioesterase II, thioesterase domain containing 1, thioesterase domain-containing protein 1, type II thioesterase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NV23
  • Protein Name
    S-acyl fatty acid synthase thioesterase, medium chain
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OLAH using anti-OLAH antibody (A05069-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U20S whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLAH antigen affinity purified polyclonal antibody (Catalog # A05069-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OLAH at approximately 36 kDa. The expected band size for OLAH is at 35 kDa.
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