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OLIG2 antibody

GTX132732
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetOLIG2
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Overview

  • Supplier
    GeneTex
  • Product Name
    OLIG2 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID10215
  • Target name
    OLIG2
  • Target description
    oligodendrocyte transcription factor 2
  • Target synonyms
    basic domain, helix-loop-helix protein, class B, 1; BHLHB1; bHLHe19; class B basic helix-loop-helix protein 1; class E basic helix-loop-helix protein 19; human protein kinase C-binding protein RACK17; OLIGO2; oligodendrocyte lineage transcription factor 2; oligodendrocyte transcription factor 2; oligodendrocyte-specific bHLH transcription factor 2; PRKCBP2; protein kinase C-binding protein 2; RACK17
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13516
  • Protein Name
    Oligodendrocyte transcription factor 2
  • Scientific Description
    This gene encodes a basic helix-loop-helix transcription factor which is expressed in oligodendroglial tumors of the brain. The protein is an essential regulator of ventral neuroectodermal progenitor cell fate. The gene is involved in a chromosomal translocation t(14;21)(q11.2;q22) associated with T-cell acute lymphoblastic leukemia. Its chromosomal location is within a region of chromosome 21 which has been suggested to play a role in learning deficits associated with Down syndrome. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • The astrocytic TRPA1 channel mediates an intrinsic protective response to vascular cognitive impairment via LIF production.
    Read more
  • Combining Hypothermia and Oleuropein Subacutely Protects Subcortical White Matter in a Swine Model of Neonatal Hypoxic-Ischemic Encephalopathy. Lee JK et al., 2021 Jan 20, J Neuropathol Exp Neurol
    Read more
  • Effect of mesenchymal stem cells on glial cells population in cuprizone induced demyelination model. Barati S et al., 2019 Jun, Neuropeptides
    Read more
  • Controlled Cortical Impact Severity Results in Graded Cellular, Tissue, and Functional Responses in a Piglet Traumatic Brain Injury Model. Baker EW et al., 2019 Jan 1, J Neurotrauma
    Read more
  • Induced Pluripotent Stem Cell-Derived Neural Stem Cell Therapy Enhances Recovery in an Ischemic Stroke Pig Model. Baker EW et al., 2017 Aug 30, Sci Rep
    Read more

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Figure 1. Western blot analysis of OLIG2 using anti-OLIG2 antibody (A02247-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human THP-1 whole cell lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OLIG2 antigen affinity purified polyclonal antibody (Catalog # A02247-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OLIG2 at approximately 40 kDa. The expected band size for OLIG2 is at 32 kDa.
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