OneScript Plus Reverse Transcriptase

Applied Biological Materials

OneScript Plus Reverse Transcriptase

9

Other Application

PCR and Proteins

Research Use Only

OneScript Plus Reverse Transcriptase is a Moloney-Murine Leukemia Virus(MMLV) Reverse Transcriptase with genetic modifications to abolish RNase H activity to achieve thermal stability. This special mutant enzyme offers higher cDNA yields, longer cDNA up to 12 kb, and is able to perform under high temperatures (50C - 55C), facilitating the elimination of secondary structures associated with GC-rich RNA templates. OneScript Plus is formulated with abms RNaseOFF Ribonuclease Inhibitor (Cat. No. G138) offering improved resistance to oxidation compared to the high oxidation-sensitive human RNase inhibitors. RNaseOFF is stable even under very low concentrations of DTT ( 1 mM), making it the best choice for ultimate RNA protection. Product Features: High thermostability at 50-55C/122-131F Synthesize 15 kb RNA Templates 10-15 minutes reaction time OneScript Plus cDNA Synthesis Kit (Cat. No. G236) also available Pair with BlasTaq 2X PCR MasterMix (Cat. No. G895) for Two-Step RT-PCR workflows

-20°C

41106300