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OPTC Antibody (OAAB18528)

OAAB18528
Aviva Systems Biology
ApplicationsWestern Blot
Product group Antibodies
TargetOPTC
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    OPTC Antibody (OAAB18528)
  • Delivery Days Customer
    23
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID26254
  • Target name
    OPTC
  • Target description
    opticin
  • Target synonyms
    oculoglycan; OPT; opticin
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C,-20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OPTC using anti-OPTC antibody (A09717-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human ARPE-19 whole cell lysates, Lane 2: monkey RF/6A whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OPTC antigen affinity purified polyclonal antibody (Catalog # A09717-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OPTC at approximately 37 kDa. The expected band size for OPTC is at 37,45 kDa.
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