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Western Blot analysis of 3T3 cells using ORC1 Polyclonal Antibody
Western Blot analysis of 3T3 cells using ORC1 Polyclonal Antibody
Western Blot analysis of 3T3 cells using ORC1 Polyclonal Antibody

ORC1 Antibody

CSB-PA003602
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetORC1
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Overview

  • Supplier
    Cusabio
  • Product Name
    ORC1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID4998
  • Target name
    ORC1
  • Target description
    origin recognition complex subunit 1
  • Target synonyms
    HSORC1, ORC1L, PARC1, origin recognition complex subunit 1, origin recognition complex, subunit 1 homolog, replication control protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13415
  • Protein Name
    Origin recognition complex subunit 1
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ORC1 using anti-ORC1 antibody (A02735-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HL-60 whole cell lysates, Lane 2: human K562 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ORC1 antigen affinity purified polyclonal antibody (Catalog # A02735-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ORC1 at approximately 100 kDa. The expected band size for ORC1 is at 100 kDa.
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