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ORP8 antibody

GTX121273
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetOSBPL8
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Overview

  • Supplier
    GeneTex
  • Product Name
    ORP8 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID114882
  • Target name
    OSBPL8
  • Target description
    oxysterol binding protein like 8
  • Target synonyms
    MST120, MSTP120, ORP8, OSBP10, oxysterol-binding protein-related protein 8, OSBP-related protein 8
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BZF1
  • Protein Name
    Oxysterol-binding protein-related protein 8
  • Scientific Description
    This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Criado Santos N, Bouvet S, Cruz Cobo M, et al. Sec22b regulates phagosome maturation by promoting ORP8-mediated lipid exchange at endoplasmic reticulum-phagosome contact sites. Commun Biol. 2023,6(1):1008. doi: 10.1038/s42003-023-05382-0
    Read this paper
  • Nguyen TT, Voeltz GK. An ER phospholipid hydrolase drives ER-associated mitochondrial constriction for fission and fusion. Elife. 2022,11. doi: 10.7554/eLife.84279
    Read this paper

Datasheet

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Figure 1. Western blot analysis of OSBPL8 using anti-OSBPL8 antibody (A08408-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human HEL whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSBPL8 antigen affinity purified polyclonal antibody (Catalog # A08408-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OSBPL8 at approximately 120 kDa. The expected band size for OSBPL8 is at 101 kDa.
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