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ProductsBack/OSBPL9 Antibody (aa686-736)

OSBPL9 Antibody (aa686-736)

LS-C762426
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetOSBPL9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    OSBPL9 Antibody (aa686-736)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Immunoprecipitation Range: 2-10 microg/mg lysate
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP, WB (1:1000 - 1:5000) Immunoprecipitation Range: 2-10 µg/mg lysate
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID114883
  • Target name
    OSBPL9
  • Target description
    oxysterol binding protein like 9
  • Target synonyms
    ORP9; ORP-9; OSBP-related protein 9; oxysterol-binding protein-related protein 9
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Paraformaldehyde-fixed, paraffin embedded Human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with OSBPL9 Polyclonal Antibody, Unconjugated (bs-17522R) at 1:200 overnight at 4°C, DAB staining.
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Figure 1. Western blot analysis of OSBPL9 using anti-OSBPL9 antibody (A09498-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OSBPL9 antigen affinity purified polyclonal antibody (Catalog # A09498-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OSBPL9 at approximately 95 kDa. The expected band size for OSBPL9 is at 90 kDa.
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