Western blot showing detection of 0.1 microg of recombinant OspB protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspB fusion protein (arrow; expected MW = 72.7 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 microm nitrocellulose. After blocking with 1% BSA-TTBS ) overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
Western blot showing detection of 0.1 microg of recombinant OspB protein. Lane 1: Molecular weight markers. Lane 2: MBP-OspB fusion protein (arrow; expected MW = 72.7 kDa). Lane 3: MBP alone. Protein was run on a 4-20% gel, then transferred to 0.45 microm nitrocellulose. After blocking with 1% BSA-TTBS ) overnight at 4oC, primary antibody was used at 1:1000 at room temperature for 30 min. HRP-conjugated Goat-Anti-Rabbit secondary antibody was used at 1:40,000 in blocking buffer and imaged on the VersaDoc MP 4000 imaging system (Bio-Rad).
OspB antibody
GTX48803
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBacteria
TargetospB
Overview
- SupplierGeneTex
- Product NameOspB Antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 1:1000. ELISA: 1:13000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ELISA
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1194340
- Target nameospB
- Target descriptionouter surface lipoprotein OspB
- Target synonymsBB_A16; BB_RS05185; outer surface lipoprotein OspB
- HostRabbit
- IsotypeIgG
- Protein IDP17739
- Protein NameOuter surface protein B
- ReactivityBacteria
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203