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Western blot analysis of OTUD3 Antibody (Center) in U251 cell line lysates (35ug/lane). This demonstrates the OTUD3 antibody detected the OTUD3 protein (arrow
Western blot analysis of OTUD3 Antibody (Center) in U251 cell line lysates (35ug/lane). This demonstrates the OTUD3 antibody detected the OTUD3 protein (arrow
Western blot analysis of OTUD3 Antibody (Center) in U251 cell line lysates (35ug/lane). This demonstrates the OTUD3 antibody detected the OTUD3 protein (arrow

OTUD3 (Center) Rabbit Polyclonal Antibody

AP53132PU-N
OriGene
ApplicationsWestern Blot
Product group Antibodies
TargetOTUD3
400 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    OTUD3 (Center) Rabbit Polyclonal Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID23252
  • Target name
    OTUD3
  • Target description
    OTU deubiquitinase 3
  • Target synonyms
    DUBA4, OTU domain-containing protein 3, OTU domain containing 3
  • Host
    Rabbit
  • Protein IDQ5T2D3
  • Protein Name
    OTU domain-containing protein 3
  • Scientific Description
    OTUD3 (Center) rabbit polyclonal antibody, Aff - Purified
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OTUD3 using anti-OTUD3 antibody (A12734). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTUD3 antigen affinity purified polyclonal antibody (Catalog # A12734) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OTUD3 at approximately 43 kDa. The expected band size for OTUD3 is at 45 kDa.
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Western Blot Positive WB detected in: U251 whole cell lysate, U87 whole cell lysate All lanes: OTUD3 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 46 kDa Observed band size: 46 kDa
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