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Overexpressing lysates

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DNA-binding activity of GATA3 was measured in OriGene over-expression lysate LY400759 and a control lysate. Three microliters of each lysate was tested with a transcription factor binding assay utilizing GATA3-specific DNA sequences. The high level of activity observed in the over-expression lysate compared to the control lysate demonstrates that the expressed GATA3 is biologically active in the lysate. Overexpression cell lysates are prepared from HEK293T cells transfected with RC201618 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
GATA3 (NM_002051) Human Over-expression LysateLC400759
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Western blot validation of overexpression lysate (Cat# LY400760) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC204215 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
G protein alpha Inhibitor 2 (GNAI2) (NM_002070) Human Over-expression LysateLC400760
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Western blot validation of overexpression lysate (Cat# LY400761) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200470 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
GNB3 (NM_002075) Human Over-expression LysateLC400761
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Western blot validation of overexpression lysate (Cat# LY400762) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201757 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
Aspartate Aminotransferase (GOT1) (NM_002079) Human Over-expression LysateLC400762
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Western blot validation of overexpression lysate (Cat# LY400763) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209199 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
GNAQ (NM_002072) Human Over-expression LysateLC400763
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Western blot validation of overexpression lysate (Cat# LY400764) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC207326 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
GNAZ (NM_002073) Human Over-expression LysateLC400764
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Western blot validation of overexpression lysate (Cat# LY400765) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200469 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
GRB2 (NM_002086) Human Over-expression LysateLC400765
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Western blot validation of overexpression lysate (Cat# LY400767) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201294 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
TFIIF (GTF2F1) (NM_002096) Human Over-expression LysateLC400767
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Western blot validation of overexpression lysate (Cat# LY400768) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC209908 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
H3.3A (H3F3A) (NM_002107) Human Over-expression LysateLC400768
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Western blot validation of overexpression lysate (Cat# LY400769) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200940 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
Glycogen synthase 1 (GYS1) (NM_002103) Human Over-expression LysateLC400769
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Western blot validation of overexpression lysate (Cat# LY400770) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC201434 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
Histone H2A.X (H2AFX) (NM_002105) Human Over-expression LysateLC400770
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Western blot validation of overexpression lysate (Cat# LY400771) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200661 using transfection reagent MegaTran 2.0 (Cat# TT210002).
Product group Lysates / Extracts
OriGene
HLAA (HLA-A) (NM_002116) Human Over-expression LysateLC400771
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